Intrinsic Properties Analysis of Multiproteases System from Marine Bacteria by Inhibitor-Subsatrate Immersion Zymography
Dan Liu,
Cui-Ling Wu,
Xing-Hao Yang,
Ri-Bang Wu,
Jiang Zhang,
Jia-Heng Huang,
Bin-Qiang Liao,
Fei Bian,
Hai-Lun He
Issue:
Volume 4, Issue 3, May 2016
Pages:
20-27
Received:
20 June 2016
Published:
21 June 2016
Abstract: Based on digital image analysis techniques and inhibitor-substrate immersing zymography, intrinsic properties of each active component in the enzymatic system secreted by marine bacteria were studied. This method provides an easy way to characterize the proteases in situ, which can be further verified by Mass spectrometry. Compared to the Folin phenol method, a traditional method used to determine proteases activities, the inhibitor-substrate immersing zymography method coupled with digital image analysis used in this study could determine caseinolytic activity and measure gelatinolytic activity at the same time. The effect on activities of extracellular proteases by inhibitor (phenylmethylsulfonyl fluoride or 1, 10-Phenanthroline) can be quantified by gray value changes of the corresponding band after electrophoretic separation. Because of its high throughput, great sensitivity, and convenient operation, inhibitor-substrate immersing zymography can be used to demonstrate the natural diversity of protein hydrolases and multienzyme expression systems. Thus, it is an effective approach to study the functional proteomics of proteases secreted by marine bacteria.
Abstract: Based on digital image analysis techniques and inhibitor-substrate immersing zymography, intrinsic properties of each active component in the enzymatic system secreted by marine bacteria were studied. This method provides an easy way to characterize the proteases in situ, which can be further verified by Mass spectrometry. Compared to the Folin phe...
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Evaluation on Nondestructive Plant Strength for Typhoon and Earthquake Areas in Taiwan
Chun-Pin Chang,
Ta-Ching Liang
Issue:
Volume 4, Issue 3, May 2016
Pages:
28-33
Received:
14 July 2016
Published:
19 July 2016
Abstract: In this study need to construct the criteria of landslide area protection for the selected vegetation materials in considering their characteristics and the soil solidities for the root system. Selection three pioneer plants, India-charcoal Trema, Formosan alder and Roxburgh sumac, doing destructive pulling resistance test. By the weight of plant above the ground, weight of root-soil, and get non-destructive resistance models. With the diameter of the tree just above the ground, were derived with multi-variable regression analysis, respectively. The models gave higher statistical regression coefficients when they were compared with the results of relative researches. The significant level factors to influence the plant pulling resistance capacity are climate and soil properties that fitting models of root strength provided the quantitative prediction on slope stability in the landslide areas.
Abstract: In this study need to construct the criteria of landslide area protection for the selected vegetation materials in considering their characteristics and the soil solidities for the root system. Selection three pioneer plants, India-charcoal Trema, Formosan alder and Roxburgh sumac, doing destructive pulling resistance test. By the weight of plant a...
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Novel Uses of Bacteriophages in the Treatment of Human Infections and Antibiotic Resistance
Shettima Abubakar,
Bello Hauwa Suleiman,
Benisheikh Ali. Abbagana,
Isa Alhaji Mustafa,
Ibn Abbas Musa
Issue:
Volume 4, Issue 3, May 2016
Pages:
34-40
Received:
7 June 2016
Accepted:
18 June 2016
Published:
28 July 2016
Abstract: As the world is facing a great challenge as a result of antibiotic resistance genes among microbial pathogens to conventional antibiotics, the exploration of the alternatives are highly essential. Scientist now struggle to eliminate the otherwise easy to treat bacterial infection as well as hospital associated infections “nosocomial infections” like methicillin resistance Staphylococcus aureus (MRSA) and vancomycin resistance enterococcus etc. Theoretically, the use of bacteriophages for treatment is simple, though, with the scope of this straightforwardness, a complex pharmacokinetics concerns exist. The basics of drug actions involve two fundamental components: pharmacodynamics and pharmacokinetics. Pharmacodynamics entails the study of the interaction of drugs with their receptors, the transduction systems to which these are linked and the changes they bring about in cells, organs, and the whole organism. While phages distribution from compartment a - b is termed phage pharmacokinetics. To achieve immediate distribution of therapeutic phages into the systemic circulation parenteral route was preferred, and oral delivery was mainly used to treat gastrointestinal infections. Human infections successfully treated using phages include, skin ulcers, wound prophylaxis, burns, gastrointestinal tract infections, respiratory tract infections and otitis etc. PhagoBioderm ® was used in the treatment of skin ulcers, and contains a cocktail of bacteriophages as antibacterial agents in patients resistant to other treatment. In conclusion, phage therapy has a greater diversity of mechanisms of action in comparison with antibiotics; it is very clear that phage therapy has many advantages, to harness this, is a challenge particularly in the face of existing rigorous regulatory practices as well as the reluctance of pharmaceuticals to invest in the field as a result of poor intellectual property and many aspects are not patentable because they are natural entities.
Abstract: As the world is facing a great challenge as a result of antibiotic resistance genes among microbial pathogens to conventional antibiotics, the exploration of the alternatives are highly essential. Scientist now struggle to eliminate the otherwise easy to treat bacterial infection as well as hospital associated infections “nosocomial infections” lik...
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