Volume 7, Issue 4, July 2019, Page: 77-81
Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by Streptomyces Sp
Totiya Ishchi, Department of Biology, Education Faculty, Jawzjan University, Sheberghan City, Afghanistan
Sibi G., Department of Biotechnology, Indian Academy Degree College-Autonomous, Bengaluru, India
Received: Oct. 8, 2019;       Accepted: Oct. 26, 2019;       Published: Oct. 31, 2019
DOI: 10.11648/j.ajbio.20190704.11      View  564      Downloads  153
Cellulases have diversity of industrial applications and their cost effective production using agroindustrial wastes by solid state fermentation poses an efficient method. Actinomycetes are considered highly valuable due to their secondary metabolites production and in this study, an attempt was made to optimize the use of ragi husk and refine the process of cellulase production by temperature mediated solid state fermentation. Actinomycetes were isolated from paper mill industry soil and cellulase producing Streptomyces sp was selected for the experiments. Ragi husk was used as substrate for solid state fermentation of cellulase and varying incubation temperatures (20°C, 25°C, 30°C, 35°C and 40°C) was considered to determine its effect on enzyme activity after 6th, 9th and 12th day of fermentation. The carboxymethyl cellualse (CMC-ase) activity was measured and the observations obtained were compared with the standard glucose curve to determine the amount of reducing sugar (µg ml-1) released. Enzyme activity was highest at 35°C and was recorded as 35.14, 45.90 and 59.56 IU ml-1 at the end of 6th, 9th and 12th day of fermentation. Highest amount of reducing sugars at a concentration of 322 µg ml-1 was released at the end of 12th day at 35°C. The results indicated that the enzyme activity was temperature dependent while using ragi husk as growth substrate under solid state fermentation.
Cellulase, Streptomyces, Solid State Fermentation, Agroindustrial Wastes, Ragi Husk, Actinomycetes
To cite this article
Totiya Ishchi, Sibi G., Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by Streptomyces Sp, American Journal of BioScience. Vol. 7, No. 4, 2019, pp. 77-81. doi: 10.11648/j.ajbio.20190704.11
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
O’Sullivan, A. C. (1997). Cellulose: the structure slowly unravels. Cellulose. 4: 173–207.
Kasana, R. C., Salwan, R., Dhar, H., Dutt, S., Gulati, A. (2008). A rapid and easy method for the detection of microbial cellulases on agar plates using gram's iodine. Curr. Microbiol. 57: 503-507.
Callow, N., Ray, C. S., Kelby, M. A., Ju, L. K. (2016). Nutrient control for stationary phase cellulase production in Trichoderma reesei Rut C30. Enzyme Microb. Technol. 82: 8-14.
Sharma, B., Agrawal, R., Singhania, R. R., Satlewal, A., Mathur, A., Tuli, D., Adsul, M. (2015). Untreated wheat straw: potential source for diverse cellulolytic enzyme secretion by Penicillium janthinellum EMS-UV-8 mutant. Bioresour. Technol. 196: 518-524.
Saini, R., Saini, J. K., Adsul, M., Patel, A. K., Mathur, A., Tuli, D., Singhania, R. R. (2015). Enhanced production by Penicillium oxalicum for bio-ethanol application. Bioresour. Technol. 188: 240-246.
Lan, T. Q., Wei, D., Yang, S. T., Liu, X. (2013). Enhanced cellulase production by Trichoderma viride in a rotating fibrous bed bioreactor. Bioresour. Technol. 133: 175–182.
Assareh, R., Zahiri, H. S., Noghabi, K. A., Aminzadeh, S., Khaniki, B. (2012). Characterization of newly isolated Geobacillus sp. T1, the efficient cellulase producer on untreated barley and wheat straws. Bioresour. Technol. 120: 99–105.
Rastogi, G., Bhalla, A., Adhikari, A., Bischoff, K. M., Hughes, S. R., Christopher, L. P., Sani, R. K. (2010). Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains. Bioresour. Technol. 101: 8798-8806.
Mohanta, Y. K. (2014). Isolation of cellulose degrading actinobacteria and evaluation of their cellulolytic potential. Bioeng. Biosci. 1: 1–5.
Cirigliano, M. N. F., Rezende, R. D. C., Oliveira, M. N. G., Pereira, P. H. F., Nascimento, R. P. D., Bon, E. P. D. S., Macrae, A., Coelho, R. R. R. (2013). Streptomyces misionensis PESB25 produces a thermoacidophilic endoglucanase using sugarcane bagasse and corn steep liquor as the sole organic substrates. Hindawi Publishing Corporation Biomed Research International (ID 584137, pp. 1–9).
Sarita, M., Arora, A., Singh, S., Nain, L. (2013). Streptomyces griseorubens mediated delignification of paddy straw for improved enzymatic saccharification yields. Bioresour. Technol. 135: 12-17.
Berdy, J. (2012). Thoughts and facts about antibiotics: Where We Are Now and Where We Are Heading. J. Antibiot., 65 (8): 385-395.
Mohseni, M., Norouzi, H., Hamedi, J., Roohi, A. (2013). Screening of antibacterial producing Actinomycetes from sediments of the Caspian Sea. Int. J. Mol. Cell Med. 2: 64-71.
Abdelmohsen, U. R., Grkovic, T., Balasubramanian, S., Kamel, M. S., Quinn, R. J., Hentschel, U. (2015). Elicitation of secondary metabolism in actinomycetes. Biotechnol. Adv. 33: 798-811.
Antonopoulos, V. T., Hernandez, M., Arias, M. E., Mavrakos, E., Ball, A. (2001). The use of extracellular enzymes from Streptomyces albus ATCC 3005 for the bleaching of Eucalyptus kraft pulp. Appl. Microbiol. Biotechnol., 57: 92-97.
Olofsson, K., Bertilsson, M., Liden, G. (2008). A short review on SSF – an interesting process option for ethanol production from lignocellulosic feedstocks. Biotechnol. Biofuels. 1: 1-14.
Singhania, R. R., Sukumaran, K. K., Patel, A. K., Larroche, C., Pandey, A. (2010). Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzyme Microb. Technol. 46: 541-549.
Thomas, L., Larroche, C., Pandey, A. (2013). Current developments in solid-state fermentation. Biochem. Eng J. 81: 146-161.
Kumar, A., Kanwar, S. S. (2012). Lipase production in solid-state fermentation (SSF): recent developments and biotechnological applications. Dyn Biochem, Process Biotechnol. Mol. Biol. 6: 13-27.
Shirling, E. B., Gottlieb, D. (1966). Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16: 313-340.
Pridham, T. G. (1965). Color and Streptomycetes. Appl. Microbiol. 13: 43-61.
Wood, T. M., Bhat, K. M. (1998). Method for measuring cellulase activities. W. A. Wood, J. A. Kellogg (Eds.), Methods in Enzymology: Cellulose and Hemicellulose, Academic Press, New York. 87-112.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
Mackenzie, C. R., Bilous, D., Schneider, H., Johnson, K. G. (1987). Induction of cellulolytic and xylanolytic enzyme systems in Streptomyces spp. Appl. Environ. Microbiol. 53: 2835-2839.
Nascimento, R. P., Junior, N. A., Pereira Jr, N., Bon, E. P. S., Coelho, R. R. R. (2009). Brewer’s spent grain and corn steep liquor as substrates for cellulolytic enzymes production by Streptomyces malaysiensis. Lett. Appl. Microbiol. 48: 529-535.
Da Vinha, F. N. M., Gravina-Oliveira, M. P., Franco, M. N., Macrae, A., Bon, E. P., Nascimento, R. P., Coelho, R. R. R. (2011). Cellulase production by Streptomyces viridobrunneus SCPE-09 using lignocellulosic biomass as inducer substrate. Appl. Biochem. Biotechnol. 164: 256-267.
Orozco, A. L., Perez, M. I., Guevara, O., Rodriguez, J., Hernandez, M., Gonzalez-Vila, F. J. (2008). Biotechnological enhancement of coffee pulp residues by solid-state fermentation with Streptomyces. Py–GC/MS analysis Journal of Analytical and Applied Pyrolysis, 81: 247-252.
Mekala, N. K., Singhania, R. R., Sukumaran, R. K., Pandey A. (2008). Cellulase production under solid-state fermentation by Trichoderma reesei RUT C30: Statistical optimization of process parameters. Appl. Biochem. Biotechnol. 151: 122-131.
Prasad, P., Singh, T., Bedi, S. (2013). Characterization of the cellulolytic enzyme produced by Streptomyces griseorubens (Accession No. AB184139) isolated from Indian soil. Journal of King Saudi University - Science. 25: 245-250.
Cadirci, B. H., Yasa, I., Kocyigit, A. (2014). Streptomyces sp. TEM 33 possesses high lipolytic activity in solid-state fermentation in comparison with submerged fermentation. Prep. Biochem. Biotechnol. 46: 23-29.
Juturu, V., Wu, J. C. (2014). Microbial cellulases: Engineering, production and applications. Renew. Sustain. Energy Rev. 33: 188-203.
Chellapandi, P., Jani, H. M. (2008). Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation. Braz. J. Microbiol. 39: 122-127.
Jaradat, Z., Dawagreh, A., Ababneh, Q., Saadoun, I. (2008). Influence of culture conditions on cellulase production by Streptomyces Sp. (Strain J2). Jordan J. Biol. Sci., 1: 141–146.
Fatokun, E. N., Nwodo, U. U., Okoh, A. I. 2016. Classical optimization of cellulase and xylanase production by a marine Streptomyces species. Appl. Sci. 6: 286.
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